• Coverage saturation
    Description

    Percentage of target-bases covered at coverage >=10x as a function of the number of mapped reads. Sequencing depth simulations were carried out by randomly selecting reads from the bam file. For each of these sets of reads, the percentage of target-covered positions was calculated. This graph aims to give an idea on how much one can improve the percentage of target-bases covered by resequencing.

    A flat curve on the right part indicates that resequencing will not improve the number of target-bases covered at 10x. A warning is issued if the curve does not tend to saturation on the right side (slope between the two last points > ). If the maximum depth provided as input is greater than the number of reads in the bam file, the last x-value corresponds to the number of reads in the bam file.